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cell culture hl60 cells  (DSMZ)


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    DSMZ cell culture hl60 cells
    Cell Culture Hl60 Cells, supplied by DSMZ, used in various techniques. Bioz Stars score: 96/100, based on 570 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cell+culture+hl60+cells/pm39945543-310-2-18?v=DSMZ
    Average 96 stars, based on 570 article reviews
    cell culture hl60 cells - by Bioz Stars, 2026-08
    96/100 stars

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    SiSTAT4 decreased STAT4 expression, while overexpressed STAT4 did conversely in transfected <t>HL60</t> and NB4 cells. (a) The expression of STAT4 in HL60 cells after transfection was examined by qPCR, and GAPDH was used as the loading gene. (b) The expression of STAT4 in HL60 cells after transfection was examined by Western blot, and GAPDH was adopted as the internal control. (c) The expression of STAT4 in NB4 cells after transfection was examined by qPCR, and GAPDH was employed as a loading gene. (d) The expression of STAT4 in NB4 cells after transfection was examined by Western blot, and GAPDH was utilized as the internal control. (e) The expression of STAT4 in HL60 cells after transfection was examined by qPCR, and GAPDH was harnessed as the reference gene. (f) The expression of STAT4 in HL60 cells after transfection was measured by Western blot, and GAPDH was exploited as the internal control. (g) The expression of STAT4 in NB4 cells after transfection was quantified by qPCR, and GAPDH was applied as the reference gene. (h) The expression of STAT4 in NB4 cells after transfection was determined by Western blot, and GAPDH was adopted as the loading control. *** P < 0.001 vs siNC; ^^^ P < 0.001 vs NC (NC: negative control, siSTAT4: siRNA targeting STAT4, siNC: negative control of siSTAT4).
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    SiSTAT4 decreased STAT4 expression, while overexpressed STAT4 did conversely in transfected <t>HL60</t> and NB4 cells. (a) The expression of STAT4 in HL60 cells after transfection was examined by qPCR, and GAPDH was used as the loading gene. (b) The expression of STAT4 in HL60 cells after transfection was examined by Western blot, and GAPDH was adopted as the internal control. (c) The expression of STAT4 in NB4 cells after transfection was examined by qPCR, and GAPDH was employed as a loading gene. (d) The expression of STAT4 in NB4 cells after transfection was examined by Western blot, and GAPDH was utilized as the internal control. (e) The expression of STAT4 in HL60 cells after transfection was examined by qPCR, and GAPDH was harnessed as the reference gene. (f) The expression of STAT4 in HL60 cells after transfection was measured by Western blot, and GAPDH was exploited as the internal control. (g) The expression of STAT4 in NB4 cells after transfection was quantified by qPCR, and GAPDH was applied as the reference gene. (h) The expression of STAT4 in NB4 cells after transfection was determined by Western blot, and GAPDH was adopted as the loading control. *** P < 0.001 vs siNC; ^^^ P < 0.001 vs NC (NC: negative control, siSTAT4: siRNA targeting STAT4, siNC: negative control of siSTAT4).
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    SiSTAT4 decreased STAT4 expression, while overexpressed STAT4 did conversely in transfected <t>HL60</t> and NB4 cells. (a) The expression of STAT4 in HL60 cells after transfection was examined by qPCR, and GAPDH was used as the loading gene. (b) The expression of STAT4 in HL60 cells after transfection was examined by Western blot, and GAPDH was adopted as the internal control. (c) The expression of STAT4 in NB4 cells after transfection was examined by qPCR, and GAPDH was employed as a loading gene. (d) The expression of STAT4 in NB4 cells after transfection was examined by Western blot, and GAPDH was utilized as the internal control. (e) The expression of STAT4 in HL60 cells after transfection was examined by qPCR, and GAPDH was harnessed as the reference gene. (f) The expression of STAT4 in HL60 cells after transfection was measured by Western blot, and GAPDH was exploited as the internal control. (g) The expression of STAT4 in NB4 cells after transfection was quantified by qPCR, and GAPDH was applied as the reference gene. (h) The expression of STAT4 in NB4 cells after transfection was determined by Western blot, and GAPDH was adopted as the loading control. *** P < 0.001 vs siNC; ^^^ P < 0.001 vs NC (NC: negative control, siSTAT4: siRNA targeting STAT4, siNC: negative control of siSTAT4).
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    SiSTAT4 decreased STAT4 expression, while overexpressed STAT4 did conversely in transfected <t>HL60</t> and NB4 cells. (a) The expression of STAT4 in HL60 cells after transfection was examined by qPCR, and GAPDH was used as the loading gene. (b) The expression of STAT4 in HL60 cells after transfection was examined by Western blot, and GAPDH was adopted as the internal control. (c) The expression of STAT4 in NB4 cells after transfection was examined by qPCR, and GAPDH was employed as a loading gene. (d) The expression of STAT4 in NB4 cells after transfection was examined by Western blot, and GAPDH was utilized as the internal control. (e) The expression of STAT4 in HL60 cells after transfection was examined by qPCR, and GAPDH was harnessed as the reference gene. (f) The expression of STAT4 in HL60 cells after transfection was measured by Western blot, and GAPDH was exploited as the internal control. (g) The expression of STAT4 in NB4 cells after transfection was quantified by qPCR, and GAPDH was applied as the reference gene. (h) The expression of STAT4 in NB4 cells after transfection was determined by Western blot, and GAPDH was adopted as the loading control. *** P < 0.001 vs siNC; ^^^ P < 0.001 vs NC (NC: negative control, siSTAT4: siRNA targeting STAT4, siNC: negative control of siSTAT4).
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    SiSTAT4 decreased STAT4 expression, while overexpressed STAT4 did conversely in transfected <t>HL60</t> and NB4 cells. (a) The expression of STAT4 in HL60 cells after transfection was examined by qPCR, and GAPDH was used as the loading gene. (b) The expression of STAT4 in HL60 cells after transfection was examined by Western blot, and GAPDH was adopted as the internal control. (c) The expression of STAT4 in NB4 cells after transfection was examined by qPCR, and GAPDH was employed as a loading gene. (d) The expression of STAT4 in NB4 cells after transfection was examined by Western blot, and GAPDH was utilized as the internal control. (e) The expression of STAT4 in HL60 cells after transfection was examined by qPCR, and GAPDH was harnessed as the reference gene. (f) The expression of STAT4 in HL60 cells after transfection was measured by Western blot, and GAPDH was exploited as the internal control. (g) The expression of STAT4 in NB4 cells after transfection was quantified by qPCR, and GAPDH was applied as the reference gene. (h) The expression of STAT4 in NB4 cells after transfection was determined by Western blot, and GAPDH was adopted as the loading control. *** P < 0.001 vs siNC; ^^^ P < 0.001 vs NC (NC: negative control, siSTAT4: siRNA targeting STAT4, siNC: negative control of siSTAT4).
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    SiSTAT4 decreased STAT4 expression, while overexpressed STAT4 did conversely in transfected HL60 and NB4 cells. (a) The expression of STAT4 in HL60 cells after transfection was examined by qPCR, and GAPDH was used as the loading gene. (b) The expression of STAT4 in HL60 cells after transfection was examined by Western blot, and GAPDH was adopted as the internal control. (c) The expression of STAT4 in NB4 cells after transfection was examined by qPCR, and GAPDH was employed as a loading gene. (d) The expression of STAT4 in NB4 cells after transfection was examined by Western blot, and GAPDH was utilized as the internal control. (e) The expression of STAT4 in HL60 cells after transfection was examined by qPCR, and GAPDH was harnessed as the reference gene. (f) The expression of STAT4 in HL60 cells after transfection was measured by Western blot, and GAPDH was exploited as the internal control. (g) The expression of STAT4 in NB4 cells after transfection was quantified by qPCR, and GAPDH was applied as the reference gene. (h) The expression of STAT4 in NB4 cells after transfection was determined by Western blot, and GAPDH was adopted as the loading control. *** P < 0.001 vs siNC; ^^^ P < 0.001 vs NC (NC: negative control, siSTAT4: siRNA targeting STAT4, siNC: negative control of siSTAT4).

    Journal: Open Medicine

    Article Title: High STAT4 expression correlates with poor prognosis in acute myeloid leukemia and facilitates disease progression by upregulating VEGFA expression

    doi: 10.1515/med-2023-0840

    Figure Lengend Snippet: SiSTAT4 decreased STAT4 expression, while overexpressed STAT4 did conversely in transfected HL60 and NB4 cells. (a) The expression of STAT4 in HL60 cells after transfection was examined by qPCR, and GAPDH was used as the loading gene. (b) The expression of STAT4 in HL60 cells after transfection was examined by Western blot, and GAPDH was adopted as the internal control. (c) The expression of STAT4 in NB4 cells after transfection was examined by qPCR, and GAPDH was employed as a loading gene. (d) The expression of STAT4 in NB4 cells after transfection was examined by Western blot, and GAPDH was utilized as the internal control. (e) The expression of STAT4 in HL60 cells after transfection was examined by qPCR, and GAPDH was harnessed as the reference gene. (f) The expression of STAT4 in HL60 cells after transfection was measured by Western blot, and GAPDH was exploited as the internal control. (g) The expression of STAT4 in NB4 cells after transfection was quantified by qPCR, and GAPDH was applied as the reference gene. (h) The expression of STAT4 in NB4 cells after transfection was determined by Western blot, and GAPDH was adopted as the loading control. *** P < 0.001 vs siNC; ^^^ P < 0.001 vs NC (NC: negative control, siSTAT4: siRNA targeting STAT4, siNC: negative control of siSTAT4).

    Article Snippet: In addition, HL60 cells were maintained in HL60 cell-specific culture medium (CM-0110; Procell).

    Techniques: Expressing, Transfection, Western Blot, Negative Control

    STAT4 silencing decreased the viability, while overexpressed STAT4 did conversely in transfected AML cells. (a–d) The viability of HL60 and NB4 cells after transfection was examined by MTT assay. * P < 0.05, *** P < 0.001 vs siNC; ^ P < 0.05, ^^^ P < 0.001 vs NC.

    Journal: Open Medicine

    Article Title: High STAT4 expression correlates with poor prognosis in acute myeloid leukemia and facilitates disease progression by upregulating VEGFA expression

    doi: 10.1515/med-2023-0840

    Figure Lengend Snippet: STAT4 silencing decreased the viability, while overexpressed STAT4 did conversely in transfected AML cells. (a–d) The viability of HL60 and NB4 cells after transfection was examined by MTT assay. * P < 0.05, *** P < 0.001 vs siNC; ^ P < 0.05, ^^^ P < 0.001 vs NC.

    Article Snippet: In addition, HL60 cells were maintained in HL60 cell-specific culture medium (CM-0110; Procell).

    Techniques: Transfection, MTT Assay

    STAT4 silencing decreased Bcl-2 level, yet increased apoptosis and Bax level in AML cells, while overexpressed STAT4 did conversely. (a and b) The apoptosis of HL60 and NB4 cells after transfection was examined by flow cytometry. (c) The expressions of Bcl-2 and Bax in HL60 cells after transfection were quantified by qPCR, and GAPDH was used as the reference gene. (d) The expressions of Bcl-2 and Bax in HL60 cells after transfection were detected by Western blot, and GAPDH was used as the loading control. (e) The expressions of Bcl-2 and Bax in NB4 cells after transfection were measured by qPCR, and GAPDH was used as the reference gene. (f) The expressions of Bcl-2 and Bax in NB4 cells after transfection were examined by Western blot, and GAPDH was used as the internal control. *** P < 0.001 vs siNC; ^^^ P < P < 0.001 vs NC.

    Journal: Open Medicine

    Article Title: High STAT4 expression correlates with poor prognosis in acute myeloid leukemia and facilitates disease progression by upregulating VEGFA expression

    doi: 10.1515/med-2023-0840

    Figure Lengend Snippet: STAT4 silencing decreased Bcl-2 level, yet increased apoptosis and Bax level in AML cells, while overexpressed STAT4 did conversely. (a and b) The apoptosis of HL60 and NB4 cells after transfection was examined by flow cytometry. (c) The expressions of Bcl-2 and Bax in HL60 cells after transfection were quantified by qPCR, and GAPDH was used as the reference gene. (d) The expressions of Bcl-2 and Bax in HL60 cells after transfection were detected by Western blot, and GAPDH was used as the loading control. (e) The expressions of Bcl-2 and Bax in NB4 cells after transfection were measured by qPCR, and GAPDH was used as the reference gene. (f) The expressions of Bcl-2 and Bax in NB4 cells after transfection were examined by Western blot, and GAPDH was used as the internal control. *** P < 0.001 vs siNC; ^^^ P < P < 0.001 vs NC.

    Article Snippet: In addition, HL60 cells were maintained in HL60 cell-specific culture medium (CM-0110; Procell).

    Techniques: Transfection, Flow Cytometry, Western Blot

    STAT4 silencing decreased the angiogenesis and VEGFA level, while overexpressed STAT4 did conversely. (a and b) The angiogenesis of HL60 and NB4 cells after transfection was examined by tube formation assay under ×100 magnification. (c) The expressions of STAT4 and VEGFA in HL60 cells after transfection were tested by qPCR, and GAPDH was used as the reference gene. (d) The expressions of STAT4 and VEGFA in HL60 cells after transfection were determined by Western blot, and GAPDH was adopted as the internal control. (e) The expressions of STAT4 and VEGFA in NB4 cells after transfection were assayed by qPCR, and GAPDH was utilized as the reference gene. (f) The expressions of STAT4 and VEGFA in NB4 cells after transfection were measured by Western blot, and GAPDH was exploited as the loading control. *** P < 0.001 vs siNC; ^^^ P < 0.001 vs NC (VEGFA: vascular endothelial growth factor A).

    Journal: Open Medicine

    Article Title: High STAT4 expression correlates with poor prognosis in acute myeloid leukemia and facilitates disease progression by upregulating VEGFA expression

    doi: 10.1515/med-2023-0840

    Figure Lengend Snippet: STAT4 silencing decreased the angiogenesis and VEGFA level, while overexpressed STAT4 did conversely. (a and b) The angiogenesis of HL60 and NB4 cells after transfection was examined by tube formation assay under ×100 magnification. (c) The expressions of STAT4 and VEGFA in HL60 cells after transfection were tested by qPCR, and GAPDH was used as the reference gene. (d) The expressions of STAT4 and VEGFA in HL60 cells after transfection were determined by Western blot, and GAPDH was adopted as the internal control. (e) The expressions of STAT4 and VEGFA in NB4 cells after transfection were assayed by qPCR, and GAPDH was utilized as the reference gene. (f) The expressions of STAT4 and VEGFA in NB4 cells after transfection were measured by Western blot, and GAPDH was exploited as the loading control. *** P < 0.001 vs siNC; ^^^ P < 0.001 vs NC (VEGFA: vascular endothelial growth factor A).

    Article Snippet: In addition, HL60 cells were maintained in HL60 cell-specific culture medium (CM-0110; Procell).

    Techniques: Transfection, Tube Formation Assay, Western Blot

    VEGFA silencing counteracted the effects of overexpressed STAT4 on promoting the viability and angiogenesis as well as inhibiting the apoptosis of AML cells. (a and b) The viability of HL60 and NB4 cells after transfection was examined by MTT assay. (c and d) The angiogenesis of HL60 and NB4 cells after transfection was determined by tube formation assay under ×100 magnification. (e and f) The apoptosis of HL60 and NB4 cells after transfection was tested by flow cytometry. * P < 0.05, ** P < 0.01 , *** P < 0.001 vs NC + vector; ^ P < 0.05, ^^ P < 0.01, ^^^ P < 0.001 vs STAT4 + vector; # P < 0.05, ## P < 0.01, ### P < 0.001 vs NC + siVEGFA (siVEGFA: siRNA targeting VEGFA, vector: negative control for siVEGFA).

    Journal: Open Medicine

    Article Title: High STAT4 expression correlates with poor prognosis in acute myeloid leukemia and facilitates disease progression by upregulating VEGFA expression

    doi: 10.1515/med-2023-0840

    Figure Lengend Snippet: VEGFA silencing counteracted the effects of overexpressed STAT4 on promoting the viability and angiogenesis as well as inhibiting the apoptosis of AML cells. (a and b) The viability of HL60 and NB4 cells after transfection was examined by MTT assay. (c and d) The angiogenesis of HL60 and NB4 cells after transfection was determined by tube formation assay under ×100 magnification. (e and f) The apoptosis of HL60 and NB4 cells after transfection was tested by flow cytometry. * P < 0.05, ** P < 0.01 , *** P < 0.001 vs NC + vector; ^ P < 0.05, ^^ P < 0.01, ^^^ P < 0.001 vs STAT4 + vector; # P < 0.05, ## P < 0.01, ### P < 0.001 vs NC + siVEGFA (siVEGFA: siRNA targeting VEGFA, vector: negative control for siVEGFA).

    Article Snippet: In addition, HL60 cells were maintained in HL60 cell-specific culture medium (CM-0110; Procell).

    Techniques: Transfection, MTT Assay, Tube Formation Assay, Flow Cytometry, Plasmid Preparation, Negative Control